EFFECT OF FETAL KIDNEY TISSUE TRANSPLANTATION ON THE COURSE OF THE SPASTICITY AND CHRONIC PAIN SYNDROME AFTER EXPERIMENTAL SPINAL CORD INJURY
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Keywords

spinal cord injury, fetal kidney tissue transplantation, spinal cord regeneration, posttraumatic spasticity syndrome, chronic pain syndrome

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Tsymbaliuk, V., Medvediev, V., Senchyk, Y., Tatarchuk, M., Draguntsova, N., & Dychko, S. (2017). EFFECT OF FETAL KIDNEY TISSUE TRANSPLANTATION ON THE COURSE OF THE SPASTICITY AND CHRONIC PAIN SYNDROME AFTER EXPERIMENTAL SPINAL CORD INJURY. Medical Science of Ukraine (MSU), 12(3-4), 21-27. Retrieved from https://msu-journal.com/index.php/journal/article/view/80

Abstract

Relevance. Creating the means of influence on angiogenesis in the area of injury is a promising, though a controversial direction of neuroengineering. The expression by tissue of injured spinal cord of angiogenic factors correlates with spasticity. In the tissue of the fetal kidney there are stem cells of mesenchymal genealogy, angiogenic growth factors.

Objective. To examine the effect of fetal kidney tissue transplantation (FKTT) on the course of the spasticity and chronic pain syndrome after experimental spinal cord injury.

Materials and methods. Animals — albino male rats (5.5 months, 300 grams, inbred line, the original strain — Wistar). There were three experimental groups formed: 1) control group (n=16), the animals of which were modelled by the spinal cord injury – left-side spinal cord hemisection at Т11 level; 2) FKTT ((n=8), where the animals after the spinal cord injury were immediately made a homotopical implantation of a fragment of the fetal (E18) kidney; 3) intact group (n = 7).

The spasticity of the posterior ipsilateral (relative to the area of the trauma of the spinal cord) limb at the level of the adnexal and tibia and knee joints was evaluated after the Ashworth scale and by the electroneuromyography. For this, impulse stimulation (5 ms, 0.2 Hz) of the sciatic nerve was performed at a distance of 5 mm from the place of the nerve output from the small pelvis. Electrical excitation was recorded in the thickness of the calf muscle. The ratio of amplitudes of the H- and M- waves in the myogram was calculated. Statistical data was processed using the STATISTICA 10.0 package.

Results. The effect of the FKTT on the dynamics of spasticity syndrome ischaracterized by a two-phase: significant potentiation during the first two weeks and stabilization on the level that is insignificantly less than control group value (1,9±0,3 vs. 2,6±0,4 Ashworth points; 24thweek). During the 2ndmonth after FKTT it was noted a positive correlation of the IHL function indicator and spasticity value, on the 1st–2nd and 9th–24th week–negative correlation. Without EKTT similar trend sin the control group have been observe dat the 1st–4th (positive correlation) and 5th–24th (negative correlation) weeks. The Н/М ratio in both groups and with respect to both hindlimbs significantly exceeded the value of the intact animals group (for the intact animals group – 34,1±3,2 %, for the IHL of the control group animals– 66,8±9,8 %, for the IHL of the "FKTT" group – 67,8±10,0 %), by the comparison between the two experimental groups and between the two hindlimbs within each group – differed insignificantly.

Conclusion. Immediate FKTT potentiates symptoms of spasticity syndrome during early period of injury, further attenuates its progression, reduces the incidence of chronic pain syndrome.

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