THE IMPACT OF SODIUM SUCCINATE ON THE ACTIVITY OF GLUTATHIONE-DEPENDENT ENZYMES IN THE BLOOD AT EXPERIMENTAL PULMONARY EMPHYSEMA
Article PDF

Keywords

pulmonary emphysema, glutathione-dependent enzymes, sodium succinate

Abstract views: 71
PDF Downloads: 34

How to Cite

Zhadan, V., & Korzhov, V. (2017). THE IMPACT OF SODIUM SUCCINATE ON THE ACTIVITY OF GLUTATHIONE-DEPENDENT ENZYMES IN THE BLOOD AT EXPERIMENTAL PULMONARY EMPHYSEMA. Medical Science of Ukraine (MSU), 12(3-4), 16-20. Retrieved from https://msu-journal.com/index.php/journal/article/view/79

Abstract

Relevance. The study of the functional activity of the glutathione-dependent enzyme system will make possibility to determine the criteria for metabolic disturbances in pulmonary emphysema and to justify the possibility of their correction with sodium succinate.

Objective. To study the effect of sodium succinate on the functional state of glutathione-dependent enzymes in the blood at experimental emphysema after administration of different doses of papain.

Materials and methods. Emphysema of the lungs in rats was modeled by a single-dose intratracheal administration of papain solution at doses of 50 mg/kg (n=16), 75 mg/kg (n=16) and 100 mg/kg (n=16) of body weight. After this 24 rats were administered 5% sodium succinate solution for 3 weeks for a therapeutic purpose subcutaneously in a dose of 100 mg / kg body weight daily. The state of the glutathione-dependent enzyme system was studied according to activity of enzymes – glutathione-reductase, glutathione-transferase and glutathione-peroxidase.

Results. The obtained data on the dynamics of changes in the activity of three glutathione-dependent enzymes showed that with emphysema, the activity of the main glutathione-dependent enzymes in the blood is decrease, indicating about decreasing of antioxidant defense processes, which can lead to systemic metabolic disturbances. There are no clear correlation between the established biochemical disorders and the dose of papain. It has been proved that the use of sodium succinate for therapeutic purposes leads to a normalization of the activity of the enzymes under study.

Conclusions. The decrease in the activity of glutathione metabolism enzymes can be considered one of the important links in the pathogenesis of emphysema. The use of sodium succinate had a stimulating effect on the activity of the main glutathione-dependent enzymes.

Article PDF

References

1. Alekseyeva L.V. [et al.]. Yantarnaya kislota – osnovnoye deystvuyushcheye veshchestvo novykh metabolicheskikh preparatov // Vrach. 2001. No. 12. P. 29-31 [in Russian].
2. Aspekty patogeneza emfizemy legkikh u bol'nykh KHOBL (COPD) / A.V. Aver'yanov [et al.] // Pul'monologiya. 2008. No. 3. P. 35-41 [in Russian].
3. Biokhimichni ta fiziolohichni zminy v krovi pry eksperymentalnii emfizemi / Ya.I. Rusinchuk [et al.] // Zdobutky klinichnoi i eksperymentalnoi medytsyny. 2016. No. 3. P. 65-67 [in Ukrainian].
4. Grigor'yeva N.Yu., Kuznetsov A.N., Sharabrin Ye.G. Khronicheskaya obstruktivnaya bolezn' legkikh: novoye o patogeneticheskikh mekhanizmakh // STM. 2011. No. 1. P. 112-116 [in Russian].
5. Kalmatov R.K., Zholdoshev S.T. Rol mekhanizmiv svobodnoradykalnoho okysnennia v patohenezi lokalnoi porazky verkhnikh dykhalnykh shliakhiv // Molodyi uchenyi. 2015. No. 10. P. 417-422 [in Ukrainian].
6. Minayeva L.V. Eksperimental'naya otsenka roli izmeneniy sistemy glutationa v realizatsii pobochnykh tsitotoksicheskikh effektov povtornogo vvedeniya tsiklofosfana: avtoref. dis. kand. med. nauk (14.00.20) / Voyenno-meditsinskaya Akademiya im. S. M. Kirova // S.-Pb., 2007. 22 p. [in Russian].
7. Rol' okislitel'nogo stressa v patogeneze khronicheskoy obstruktivnoy bolezni legkikh / L.Ye. Muravleva [et al.] // Uspekhi sovremennogo yestestvoznaniya. 2012. No. 9. P. 12-16 [in Russian].
8. Serebrovskaya M.T., Shishkanov V.V., Aytukov M.T. Surfaktantnaya sistema legkikh pri eksperimental'noy papainovoy emfizeme // Pat. fiziologiya i eksperim. Terapiya // 1982. No. 5. P. 63-64 [in Russian].
9. Soodayeva S. K. Okislitel'nyy stress i antioksidantnaya terapiya pri zabolevaniyakh organov dykhaniya // Pul'monologiya. 2006. No. 5. P. 122-126 [in Russian].
10. Chervonskaya G.P., Pankratova G.P., Mironova L.A. Etika mediko-biologicheskogo eksperimenta v doklinicheskikh issledovaniyakh // Toksikologicheskiy vestnik. 1998. No. 3. P. 2-8 [in Russian].
11. Shmelev Ye.I. Svezhiy vzglyad na KHOBL (COPD). Atmosfera // Pul'monologiya i allergologiya. 2011. No. 4. P. 51-54 [in Russian].
12. Fischer B.M., Pavlisko E., Voynow J.A. Pathogenic triad in COPD: oxidative stress, protease-antiprotease imbalance, and inflammation // Int. J. Chron. Obstruct. Pulmon. Dis. 2011. Vol. 6. P. 413-421.
Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.