DYNAMIC OF CONNECTIVE-TISSUE CELL REACTIONS OF THE SKIN OF WHITE RATS WITH BURN INJURY AND PERSISTENT UNCORRECTED HYPERGLYCEMIA
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Keywords

cellular reactions of the connective tissue, burn injury, persistent uncorrected hyperglycemia

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Kolesova , N., Natrus, L., Litvinenko, V., Sukhareva, N., & Ryzhko, I. (2017). DYNAMIC OF CONNECTIVE-TISSUE CELL REACTIONS OF THE SKIN OF WHITE RATS WITH BURN INJURY AND PERSISTENT UNCORRECTED HYPERGLYCEMIA. Medical Science of Ukraine (MSU), 12(3-4), 4-10. Retrieved from https://msu-journal.com/index.php/journal/article/view/77

Abstract

Relevance. One of the main function of the connective tissue is the function of maintenance of homeostasis and homeokinesis in the organism. Cooperative engagement between all cellular and noncellular elements plays the key role in it. The problem of running of cellular reactions in the process of injury repair with the following performance of improvement of treatment options is essential in the medicine.

Objective: to investigate the mechanisms of development of tissue reactions, the elements of cell cooperation, specific features of regenerative process during local burn injury and carbohydrate metabolic imbalance.

Materials and methods. Conducted pathomorphological and histochemical studies of dynamic (3,7,14,21 days) of burn wound healing of the skin of white sexually mature rat-males of lines Wistar white rats 180-210 g without somatic pathology (n=30) as control group and rats, which have of persistent uncorrected hyperglycemia (n=30). The sample of persistent uncorrected hyperglycemia was reproduced by one-time injection of streptozotocini with dose 50 mg/kg of intraperitoneal. The burn wound of skin was represented with the help of application of metal plate to the side surface of white rat’s bodies, with the reproduction of burn of II degree – superficial dermal burn. For the pathomorphological and histochemical studies flaps of skin from lateral region of the body were taken. The common methods of pathomorphological analysis were used. The density of fibroblasts, macrophages and neutrophils was calculated in the square 130*130 mkm of histological slice of the skin with the thickness 7 mkm. The histochemical investigation of oxidoreductase activity was carried out on the cryostat section (10 mkm). The histochemical indicator of oxidoreductase activity was calculated by semiquantitative method. Digital material was processed by methods of variation statistics with the help of Student's test.

Results and discussion. The studies of influence of persistent uncorrected hyperglycemia on repair of burn injury showed that under these conditions the amount of cells of inflammatory infiltration increases and the amount of fibroblasts decreases. It is important to emphasize that the maximum changes in the level of leukocyte-macrophage infiltration and the content of fibroblast cells are observed in 7 days after application of burn injury.

Conclusions. The obtained results allow to assume that during the persistent uncorrected hyperglycemia it is reasonable to use pharmaceuticals with  antihypoxic and antioxidative action in the protocols of the treatment process .

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