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aminomethanesulfonic acids, filtering fibrous materials, antimicrobial activity, Staphylococcus aureus strains with different levels of antibiotics resistance, scanning electron microscopy

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Hrydina , T., Khoma , R., Fedchuk , A., Hruzevskyi , O., Shevchuk, H., & Ishkov , Y. (2023). ANTIMICROBIAL PROPERTIES OF FILTERING FIBROUS MATERIALS IMPREGNATED BY AMINOMETHANESULPHONIC ACIDS. Medical Science of Ukraine (MSU), 19(1), 89-97.


Background. The spread of a new strain of SARS-CoV-2 and the pandemic that caused it has led to huge changes around the world. So, it reminded us again about the importance of developing measures for the prevention of infections that transmitted by air droplets.

Aim: investigation of the antimicrobial activity of aminomethanesulfonic acid (AMSA) and its derivatives (N-(2-hydroxyethyl)-(HEAMSA), N-benzyl-(BnAMSA) and N-(tert-butyl)-(t-BuAMSA)) which impregnated on filtering fibrous material and can be used for the manufacture of anti-aerosol elements of individual respiratory protection against strains of Staphylococcus aureus with different level of antibiotics resistance.

Materials and methods. The standard method of Kirby and Bauer disks is used for the investigation of the specific activity of antimicrobial drugs. The 24-hour cultures of microorganisms that contained (1.2+0.2)x109 CFU/ml were diluted according to the turbidity standard. The results were detected after 18-20 hours of incubation at 37°C. The 0.5 cm diameter filter fibrous discs contained the following compounds: AMSA, HEAMSA, BzAMSA, t-BuAMSA and streptocide (sulfanilamide) as a reference with active compound content (Q) at a final concentration of 0.047 and 0.236 mmol/g.

Results. All test samples with aminomethanesulfonic acids at a final concentration of 0.236 mmol/g that were applied to the filter fibrous material had a higher level of inhibition of the growth of the microorganisms than the prototype using streptocide (sulfanilamide) e against the strains of Staphylococcus aureus 2781 and Staphylococcus aureus Kunda.

Conclusions. Samples based on HEAMSA showed the greatest antimicrobial activity against the studied strains of Staphylococcus aureus from all research samples of filter fibrous material with YAMSA. They were characterized by the maximum hydrophilicity and the minimum value of the empirical pKa function lgPow. AMSA and HEAMSA were characterized by the smallest particle sizes on the surface of lavsan fiber according to electron microscopy analysis.  This provides the largest boundary of contact between the phases of these biocides in the composition of the filtering fibrous material with bio aerosols during respiratory air purification.
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Pompilio A., Di Bonaventura G. Ambient air pollution and respiratory bacterial infections, a troubling association: epidemiology, underlying mechanisms, and future challenges. Critical Reviews in Microbiology. 2020; 46(5): 600-630. DOI: 10.1080/1040841X.2020.1816894

Ashour N.A., Elmaaty A.A., Sarhan A.A., Elkaeed E.B., Moussa A.M., Erfan I.A., Al-Karmalawy A.A. A Systematic Review of the Global Intervention for SARS-CoV-2 Combating: From Drugs Repurposing to Molnupiravir Approval. Drug Design, Development and Therapy. 2022; 16: 685-715. DOI: 10.2147/dddt.s354841

Beloborodova N.V., Zuev E.V., Zamyatin M.N., Gusarov V.G. Causal Therapy of COVID-19: Critical Review and Prospects. General Reanimatology. 2020; 16(6): 65-90. DOI: 10.15360/1813-9779-2020-4-0-1

Auwaerter P.G. Coronavirus COVID-19 (SARS-CoV-2). Johns Hopkins ABX Guide. The Johns Hopkins University. 2023. Johns Hopkins Guide. URL:

Noruzi A., Gholampour B., Gholampour S.,Jafari S., Farshid R., Stanek A., Saboury A.A. Current and Future Perspectives on the COVID-19 Vaccine: A Scientometric Review. Journal of Clinical Medicine. 2022; 11(3): 750. DOI: 10.3390/jcm11030750

Srivastava N., Saxena S.K. Prevention and Control Strategies for SARS-CoV-2 Infection. In: Saxena S. (eds) Coronavirus Disease 2019 (COVID-19). Medical Virology: From Pathogenesis to Disease Control. Springer, Singapore. 2020. P. 127-140. DOI: 10.1007/978-981-15-4814-7_11.

Clase C.M., Fu E.L., Ashur A., Beale R.C.L., Clase I.A., Dolovich M.B., Jardine M.J., Joseph M., Kansiime G., Mann J.F.E., Pecoits-Filho R., Winkelmayer W.C., Carrero J.J. Forgotten Technology in the COVID-19 Pandemic: Filtration Properties of Cloth and Cloth Masks-A Narrative Review. Mayo Clinic Proceedings. 2020; 95(10): 2204-2224. DOI: 10.1016/j.mayocp.2020.07.020

Infection prevention and control during health care when coronavirus disease (‎COVID-19)‎ is suspected or confirmed: interim guidance. World Health Organization. ‎2021. 12.07.2021. World Health Organization. URL:

Ueki H., Furusawa Y., Iwatsuki-Horimoto K, Imai M., Kabata H., Nishimura H., Kawaokaa Y. Effectiveness of Face Masks in Preventing Airborne Transmission of SARS-CoV-2. mSphere. 2020; 5(5). e00637-20. DOI: 10.1128/mSphere.00637-20

Chan N.C., Li K., Hirsh J. Peripheral Oxygen Saturation in Older Persons Wearing Nonmedical Face Masks in Community Settings. Journal of the American Medical Association. 2020; 324(22): 2323-2324. DOI: 10.1001/jama.2020.21905

Shein S.L., Whitticar S., Mascho K.K., Pace E., Speicher R., Deakins K. The effects of wearing facemasks on oxygenation and ventilation at rest and during physical activity. PLoS One. 2021; 16(2). e0247414. DOI: 10.1371/journal.pone.0247414

Samannan R., Holt G., Calderon-Candelario R., Mirsaeidi M., Campos M. Effect of Face Masks on Gas Exchange in Healthy Persons and Patients with Chronic Obstructive Pulmonary Disease. Annals of the American Thoracic Society. 2021; 18(3): 541-544. DOI: 10.1513/AnnalsATS.202007-812RL

Hill W.C., Hull M.S., MacCuspie R.I. Testing of Commercial Masks and Respirators and Cotton Mask Insert Materials using SARS-CoV-2 Virion-Sized Particulates: Comparison of Ideal Aerosol Filtration Efficiency versus Fitted Filtration Efficiency. Nano Letters. 2020; 20(10): 7642-7647. DOI: 10.1021/acs.nanolett.0c03182

Hrydina T.L., Khoma R.E., Ennan A.A.-A., Fedchuk A.S., Hruzevskyi O.A. [Investigations of the antimicrobial activity of aminomethanesulfonic acids against strains of Staphylococcus aureus with different antimicrobial susceptibility]. Zaporozhye Medical Journal. 2019; 21(2): 234-239. [in Ukrainian]. DOI: 10.14739/2310-1210.2019.2.161502

Khoma R.E., Gelmboldt V.O., Ennan A.A., Gridina T.L., Fedchuk A.S., Lozitsky V.P., Rakipov I.M., Vladyka A.S. Synthesis and Antioxidant and Anti-Influenza Activity of Aminomethanesulfonic Acids. Pharmaceutical Chemistry Journal. 2019; 53(5): 436-439. DOI: 10.1007/s11094-019-02016-w

Khoma R.E., Baumer V.N., Ennan A.A, Antonenko P.B., Godovan V.V., Dlubovskiy R.M. Synthesis, crystal structure, and spectral characteristics of N-(n-propyl)aminomethanesulfonic acid. Acute toxicity of aminomethanesulfonic acid and its N-alkylated derivatives. Voprosy Khimii i Khimicheskoi Tekhnologii. 2019; (6): 255-262. DOI: 10.32434/0321-4095-2019-127-6-255-262

Ministry of Health of Ukraine order dated [On approval of methodical instructions “Determining the sensitivity of microorganisms to antibacterial drugs”]. 04.05.2007. No. 167. [in Ukrainian]. URL:

Kirby-Bauer Disk Diffusion Susceptibility Test Protocol. URL:

Chemistry Software, HyperChem, Molecular Modeling. URL:

Khoma R.E. Thermodynamics of the dissociation of aminomethanesulfonic acid and its N-substituted derivatives in aqueous solutions at 293–313 K. Russian Journal of Physical Chemistry. 2017; 91(1): 76-79. DOI: 10.1134/S003602441701012

Remkoa M., Von der Lieth C.-W. Theoretical study of gas-phase acidity, pKa, lipophilicity, and solubility of some biologically active sulfonamides. Bioorganic Medical Chemistry. 2004: 12(20); 5395–5403. DOI: 10.1016/j.bmc.2004.07.049

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