EVALUATION OF ANTIULCEROGENIC EFFECT OF CRYOCONSERVATED PLACENTA EXTRACT ON THE MODEL OF ETHANOL-PREDISONOLIC LESIONS OF THE GASTRIC MUCOSA
Article PDF (Українська)

Keywords

cryopreserved placenta extract, gastric ulcer, antiulcer therapy, gastric mucosa

Abstract views: 56
PDF Downloads: 29

How to Cite

Koshurba, I., Hladkykh, F., & Chyzh , M. (2022). EVALUATION OF ANTIULCEROGENIC EFFECT OF CRYOCONSERVATED PLACENTA EXTRACT ON THE MODEL OF ETHANOL-PREDISONOLIC LESIONS OF THE GASTRIC MUCOSA. Medical Science of Ukraine (MSU), 18(2), 3-9. https://doi.org/10.32345/2664-4738.2.2022.01

Abstract

Relevance. Gastric and duodenal ulcers occur in 6-10% of the adult population, and mortality from peptic ulcers ranges from 5 to 15%. Conservative treatment of this disease almost always involves the use of antisecretory and antibacterial drugs. Existing treatment regimens for peptic ulcer disease, focused on key pathogenetic mechanisms, do not take into account the individual adaptive and trophological characteristics of patients. As a potential antiulcer agent, our attention was drawn to the cryopreserved placenta extract ("Cryocell-cryoextract placenta"). It eliminates the ulcerogenic effect of anti-inflammatory drugs of non-steroidal nature, without affecting their therapeutic efficacy.

Objective: to characterize the anti-ulcerogenic effect of therapeutic and prophylactic use of cryopreserved placenta extract in the model of alcohol-prednisolone gastric lesions in rats.

Methods. The study was performed on 28 male rats weighing 200-220 g. they were randomized into four groups: I (n = 7) – intact rats, II (n = 7) – control – rats with model pathology (alcohol-prednisolone gastric lesion) without treatment, III (n = 7) – rats with alcohol-prednisolone gastric lesions, which were injected with cryoextract placenta (0.16 ml / kg body weight, intramuscularly), IV (n = 7) – rats with alcohol-prednisolone gastric lesions, which were administered the reference drug esomeprazole, proton pump inhibitor (50 mg / kg , intragastric). Gastric lesions in rats were simulated by intragastric single administration of prednisolone (20 mg/kg) dissolved in 80.0% ethanol (0.6 ml/100 g body weight). Cryoextracts of the placenta were administered intravenously in a prophylactic mode – 1 time per day for 4 days before and 1 time 60 minutes after the introduction of ethanol-prednisolone mixture. In 24 h. after administration of the mixture, rats were removed from the experiment and the size of the stomach (bloating) and the presence of adhesions with adjacent organs were evaluated macroscopically by the following criteria: erosions and hemographies, hyperemia, edema and mucosal fold disorders. For each group, the percentage of experimental animals was calculated according to these characteristics and the average value of their severity. The values ​​of the ulcer index were calculated for each group.

Results. The study showed that the introduction of placental cryoexact as well as the reference drug esomeprazole led to a statistically significant (p<0.05) reduction of three times the prevalence of gastric ulcers in rats after administration of alcohol-prednisolone mixture. The use of placental cryoextract in the treatment-and-prophylactic regimen led to a statistically significant (p<0.05) decrease in the value of the ulcer index in 13 times relative to the control rats, and the average score of macroscopic assessment of gastric mucosa was 3.5 times lower and was 1.1±0.24 and 3.9±0.26, respectively. In animals treated with esomeprazole 2 times more moderate (2 [0.5; 3] points) hyperemia of the central nervous system (57.1% and 28.6%, respectively) and several times more moderate (2 [0; 2]). points) edema of the secondary school. In addition, edema of the central nervous system in rats administered esomeprazole led to a clear (3 [2; 3] points) violation of the folding of the central nervous system in 71.4% of rats. In contrast to the use of esomeprazole, placental cryoextract more significantly leveled the hyperemia induced by the introduction of alcohol-prednisolone mixture and edema of the gastric mucosa, which were observed in only 28.6% and 14.3% of rats, respectively.

Conclusions. Therapeutic and prophylactic use of placental cryoextract was accompanied by statistically significant (p <0.05) comparable to esomeprazole antiulcer efficacy in the model of ethanol-prednisolone gastric lesion and amounted to 92.3%.

https://doi.org/10.32345/2664-4738.2.2022.01
Article PDF (Українська)

References

Sverden E, Agreus L, Dunn JM, Lagergren J. Peptic ulcer disease. British Medical Journal. 2019;367:l5495. DOI: 10.1136/bmj.l5495

View at:

Publisher Site: https://www.bmj.com/content/367/bmj.l5495/related

PubMed: https://pubmed.ncbi.nlm.nih.gov/31578179/

Shell EJ. Pathophysiology of peptic ulcer disease. Physician Assistant Clinics. 2021;6(4):603-11. DOI: 10.1016/j.cpha.2021.05.005

View at:

Publisher Site: https://physicianassistant.theclinics.com/article/S2405-7991(21)00047-5/fulltext

Bereda G. Peptic Ulcer Disease: Definition, Pathophysiology, and Treatment. Journal of Biomedical and Biological Sciences. 2022;1(2):1-10.

View at:

Researchgate: https://www.researchgate.net/publication/360457776_Definition_Pathophysiology_and_Treatment

Hladkykh FV. [Antiulcer activity of placental cryoextract in experimental indomethacin-induced ulcerogenesis]. Lviv Medical Journal. 2021;27(3-4):67-82. [in Ukrainian]. DOI: 10.25040/aml2021.3-4.067

View at:

Researchgate:

https://www.researchgate.net/publication/358214817_Protivirazkova_aktivnist_krioekstraktu_placenti_pri_eksperimentalnomu_indometacin-indukovanomu_ulcerogenezi

Hladkykh FV. Gastrocytoprotective properties of cryopreserved placenta extract in combined action of low temperatures and inhibition of cyclooxygenase. Acta Facultatis Medicae Naissensis. 2022;39(1):48-56. DOI: 10.5937/afmnai39-33036

View at:

Publisher Site: https://scindeks.ceon.rs/article.aspx?artid=0351-60832201048H

URL: https://publisher.medfak.ni.ac.rs/AFMN_1/2022/1-2022/full%20text/5Gastrocytoprotective%20Properties%20F.V.Hladkykh.pdf

Hladkykh FV. [Anti-inflammatory properties of diclofenac sodium on the background of combined use with cryopreserved placenta extract in the experiment]. Problems of cryobiology and cryomedicine. 2021;31(4):364-7. [in Ukrainian]. DOI: 10.15407/cryo31.04.364

Holtsev AN, Yurchenko TN, ed., Blazhko EV, Bobyreva LE, Heraskyna LR, Hryshchenko VY, Hubyna-Vakulyk HY, Dvornyk YL, Evtereva YA, Zhdan VN, Zvarych PR, Kapustianskaia AA, Kuzmyna YIu, Lypyna OV, Lomakova YV, Lutsenko NS, Muryzyna YIu, Plotnykova VN, Prokopiuk VIu, Prokopenko OS, Reznykova VA, Strona VY, Strona DV, Tryfanov VIu, Feskova AM, Feskova YA, Shepytko VY, Shepytko KV. [Placenta: cryopreservation, clinical use]. Kharkiv: Brovyn AV, 2013. 268 p. [in Ukrainian].

Pan SY, Chan MKS, Wong MBF, Klokol D, Chernykh V. Placental therapy: An insight to their biological and therapeutic properties. Journal of Medicine and Therapeutics. 2017;1(3):1-6. DOI: 10.15761/JMT.1000118

View at:

Publisher Site: https://www.oatext.com/placental-therapy-an-insight-to-their-biological-and-therapeutic-properties.php

Pogozhykh O, Prokopyuk V, Figueiredo C, Pogozhykh D. Placenta and placental derivatives in regenerative therapies: experimental studies, history, and prospects. Stem Cells Int. 2018;2018:1-14. DOI: 10.1155/2018/4837930

View at:

Publisher Site: https://www.hindawi.com/journals/sci/2018/4837930/

PubMed: https://pubmed.ncbi.nlm.nih.gov/29535770/

PubMed Central: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5822788/

Stefanov OV. [Preclinical studies of drugs: guidelines]. Kyiv: Avicenna, 2001. 528 p. [in Ukrainian].

View at:

Publisher Site: https://hi.de1lib.org/book/3139988/12a2a7

Vogel HG. ed. Drug Discovery and Evaluation: Pharmacological Assays Berlin, Heidelberg: Springer Berlin Heidelberg, 2008. 2071 p.

View at:

Publisher Site: https://link.springer.com/referencework/10.1007/978-3-540-70995-4

Rybolovlev UR, Rybolovlev RS. [Dosage of substances for mammals by constants of biological activity]. Reports of the USSR Academy of Sciences. 1979; 247(6):1513-6. [in Russian]

Xie W, Huang X, Chen R, Chen R, Li T, Wu W, Huang Z. Esomeprazole alleviates the damage to stress ulcer in rats through not only its antisecretory effect but its antioxidant effect by inactivating the p38 MAPK and NF-κB signaling pathways. Drug Design, Development and Therapy. 2019; 22(13):2969-84. DOI: 10.2147/DDDT.S193641

View at:

Publisher Site: https://www.dovepress.com/esomeprazole-alleviates-the-damage-to-stress-ulcer-in-rats-through-not-peer-reviewed-fulltext-article-DDDT

PubMed: https://pubmed.ncbi.nlm.nih.gov/31686780/

PubMed Central: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6709796/

Hladkykh FV, Stepanuk NG, Vernygorodsky SV. [Macro- and microscopic study of the effect of 2-phenyl-3-carbetoxy-4-dimethylaminomethyl-5-oxybenzofuran hydrochloride (vinboron) on gastrotoxicity ibuprofen in experimental rheumatoid arthritis in rats]. Path of Science. 2017;10:7001-18. [in Ukrainian]. DOI: 10.22178/pos.27-8

View at:

Publisher Site: https://pathofscience.org/index.php/ps/article/view/399

Index Copernicus: https://journals.indexcopernicus.com/search/journal/issue?issueId=140623&journalId=43413

URL: https://journals.indexcopernicus.com/api/file/viewByFileId/164819

Feshchenko HI, Oleshchuk OM, Marchyshyn SM, Koshova OYu. The study of the antiulcer effect of the lyophilized extract of fireweed herb on the model of ethanol-prednisolone stomach damage in rats. Clinical pharmacy. 2019; 23(1):11-8. DOI: 10.24959/cphj.19.1487

View at:

Publisher Site: http://cphj.nuph.edu.ua/article/view/cphj.19.1487

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.