Relevance. The search for new forms of herbal medicine is an important area of modern biotechnology, pharmacology and pharmacy. More and more attention is paid to the development of extracts from plant materials, since the extraction products provide the maximum content of biologically active substances, which increases the rationality of their use, due to the accuracy of dosing, the therapeutic effect is increased, the period of use increases and storage conditions are simplified. The extraction of the fruit mixture of Citrullus сolocynthis in the Soxhlet apparatus was modified by reducing the procedure time 5-10 times and improving the processing of raw materials by using a press at the same time and increasing the temperature, or even by modifying the apparatus.
Objective: to study the composition of FA in an aqueous solution of the dry extract of Citrullus сolocynthis when modifying the extraction mixture in the Soxhlet apparatus by increasing the temperature and technological reduction of processing time.
Materials and methods. We carried out the determination and comparison of the composition of FA’s by gas-liquid chromatography of all parts of the fruit of Citrullus сolocynthis and in an aqueous solution of its dry extract with various concentrations of the substance.
Results. Chromatographic analysis showed that all parts of the fetus Citrullus colocynthis are promising sources of saturated and unsaturated FA. In all parts of the fruit of Citrullus colocynthis, linoleic acid prevails in quantitative content. The modification of the extraction of the mixture in the Soxhlet apparatus by increasing the temperature and technological reduction in the processing time of the raw materials led to the production of a substance with a new composition and FA content. The ratio of unsaturated / saturated FA in the resulting dry extract is 5 times greater than the similar ratio separately in seeds, pulp and shell. At the same time, the distribution of separate unsaturated and polyunsaturated FA’s in the dry extract solution is fairly uniform. The relative amount of linoleic FA in the dry extract was smaller, and the linolenic FA was greater than in the seeds and pulp of the fruit.
Conclusions. Modifying the extraction of the mixture in the Soxhlet apparatus by increasing the temperature and technological reduction of the processing time did not lead to complete degreasing of the substrate and allowed to obtain a dry extract of Citrullus solvent with content of microdoses of the FA, their balanced (uniform) in amount, and predominance of 5 times the amount of unsaturated polyunsaturated FA over the amount of saturated. We assume that such a redistribution of FA, and especially their micro doses, can be the basis for creating medicines with more effective effects on the body.
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