IMPROVEMENT OF THE ANALYTICAL CONTROL FOR APPLICATION OF PESTICIDES IN THE SYSTEM OF CHEMICAL PROTECTION OF CHICKPEA
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Keywords

pesticides, chickpea grain, limit of quantification, high performance liquid chromatography (HPLC)

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KorshunО., Lipavska А., Milokhov , D., Аvramchuk А., & Omelchuk , S. (2021). IMPROVEMENT OF THE ANALYTICAL CONTROL FOR APPLICATION OF PESTICIDES IN THE SYSTEM OF CHEMICAL PROTECTION OF CHICKPEA. Medical Science of Ukraine (MSU), 17(2), 96-101. https://doi.org/10.32345/2664-4738.2.2021.14

Abstract

Relevance. To date, there are no approved methods in Ukraine for the determination of azoxystrobin, thiabendazole, fludioxonil and pyridate in grain chickpeas. Therefore, there is a need for scientific substantiation of the method selection, the development of conditions for sample preparation of chickpea grain, qualitative identification and quantification of pesticides in this matrix used in protection system of chickpea was given in the article, which will allow to control the established hygienic standards of pesticides and minimize pesticide negative impact on population health and the environment.

Objective of the research is to develop methods for the determination of azoxystrobin, thiabendazole, fludioxonil and pyridate in grain chickpea.

Materials and methods. Chromatographic analysis was performed by Shimazu (Japan) liquid chromatographs using ultraviolet and fluorescent detection.

The package of IBM SPSS StatisticsBase v.22 and MS Exel statistical programs was used for statistical processing of results.

Results. Optimal conditions of sample preparation of chickpea grain and chromatographic determination of azoxystrobin, fludioxonil, thiabendazole and pyridate (as the sum of pyridate and its metabolite – pyridafol) with the limits of quantitative determination of 0.1; 0.1; 0.01 and 0.05 mg/kg, respectively, wеre developed to provide the determination of the analyzed compounds at the required level (the measure of correctness is (72–75)%) and the control of the established hygienic standards of these compounds in chickpea grain.

Conclusions. Developed methods for determination of azoxystrobin, fludioxonil, thiabendazole and pyridate in chickpea grain by high-performance liquid chromatography allow to control the established hygienic standards, to obtain representative information on the content of pesticide residues, which is a prerequisite for risk assessment of plant protection products.

https://doi.org/10.32345/2664-4738.2.2021.14
Article PDF (Українська)

References

Kalenskaya SM, Novitskaya NV, Netupskaya IT. [The formation of the chickpea crop under the influence of elements of cultivation technology]. Bulletin of the Poltava State Agrarian Academy. 2012; 2: 21-5. [in Ukrainian] DOI: 10.31210/visnyk2012.02.03

View at:

Publisher site: http://journals.pdaa.edu.ua/visnyk/article/view/542

URL: https://www.pdaa.edu.ua/sites/default/files/visnyk/2012/02/021.pdf

Stolyarov BV, Savinov IM, Wittenberg AG et al. [Practical gas and liquid chromatography: textbook]. St. Petersburg: Publishing house of St. Petersburg. un-ta, 1998. 612 p. [in Russian]

View at:

Publisher site: https://www.twirpx.com/file/177874/

Kudris I.V., Kulikov A.Yu. [Evaluation of the variability of the relative retention times when using chromatographic columns grafted with C18 groups]. Methods for detecting chemistry analysis. 2014;9(1): 12-8. [in Russian]

View at:

Publisher site:

PubMed: http://nbuv.gov.ua/UJRN/Moca_2014_9_1_4

URL: http://chembull.univer.kharkov.ua/archiv/2014/09.pdf

The PPDB A to Z List of Pesticide Active Ingredients. Pesticide Properties University of Hertfordshire.

View at:

Publisher site: http://sitem.herts.ac.uk/aeru/ppdb/en/atoz.htm

Klisenko MA, Alexandrova LG, Demchenko VF, Makarchuk TL Analytical chemistry of pesticide residues: textbook. Кyiv: ECOGINTOX, 1999. 238 p. [in Ukrainian]

International standard ISO 0 8466-1: 1990 (E). [Water quality - Calibration and evaluation of analytical methods to determine performance. Part 1: Statistical processing of a linear calibration function]. 10 p. [in Russian]

View at:

Publisher site: https://standartgost.ru/g/ISO_8466-1:1990

[On the use of norms of accuracy and correctness of measurements in the control of the content of chemicals in food raw materials, food and environmental objects and the correspondence between the values of MDR and MPC and the limits of analytical determination of chemicals] / Resolution of the Ministry of Health of Ukraine № 20 of April 20, 1999. [in Ukrainian]

Bublik LI, Gavrilyuk LL. Methods of monitoring and control of pesticide residues in agrocenoses. Plant protection and quarantine. 2014; 60.: 53-66. [in Ukrainian]

View at:

PubMed: http://nbuv.gov.ua/UJRN/Zikr_2014_60_9

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