FREEZING DYNAMICS 10% CALCIUM CHLORIDE SOLUTION WITH LIQUID NITROGEN IN VITRO
Article PDF

Keywords

cryodestruction, local cryoeffect, enhancement of cryodestruction, 10 % solution of calcium chloride

How to Cite

Dronov, O., KovalskaІ., Kozachuk, Y., Lukyanova, N., Khomenko, D., & Bakunets, P. (2018). FREEZING DYNAMICS 10% CALCIUM CHLORIDE SOLUTION WITH LIQUID NITROGEN IN VITRO. Medical Science of Ukraine (MSU), 14(3-4), 23-28. https://doi.org/10.32345/2664-4738.3-4.2018.03

Abstract

Relevance. Cryosurgical method is method of  ultra-low temperatures local  application for complete destruction of the pathological focus.

Nowadays, question of cryosurgery  radicality remains unsolve, because of inability to achieve lethal for tumor cells temperatures  in  depth from cryoapplicator.That`s why, attempts of cryodestruction improving  are still keep going by developing methods of ultra-low temperatures cytodestructive effect  potentiation,  particulary, by their combination with chemical agents.

Objective of the study was to determine the peculiarities of changes in temperature indices 10 % CaCl2 solution freezing in comparison with 0,9 % sodium chloride solution (0,9 % NaCl) in vitro.

Materials and methods. Dynamic of low-temperatures changes were measured at control depths of 3, 8, 13, and 18 mm in 10% СаСl2 and 0.9% NaCl solutions by a four-channel measuring thermocouple complex KIIT-4. Cryo-tool of the original design was used for freezing. Cryoagent - liquid nitrogen (temperature -1800C). The temperature indices were detected during 10-minute exposure to liquid nitrogen.

Results. During cryogenic exposure for 10 minutes at a distance of 13 and 18 mm from the cryoprobe, the mean temperatures in 10 % CaCl2 were significantly lower than 0,9 % NaCl (p <0,05). At control depths from 5th and 10th minute of exposure in 10 % CaCl2 the temperatures were significantly lower than 0,9 % NaCl (p <0,001).

Conclusion. Consequently, 10 % CaCl2 can potentiate in vitro freezing processes and can be used to further improvemnt cryosurgery efficiency in vivo.

https://doi.org/10.32345/2664-4738.3-4.2018.03
Article PDF

References

Chzhao A.V., Ionkin D.A., Zhavoronkova O.I., Shurakova A.B., Kungurcev S.V. Kombinacija metodov lokalnoj destrukcii pri pervichnom i metastaticheskom rake pecheni [Combination of local destruction methods in primary and metastatic liver cancer] // Rossijskij onkologicheskij zhurnal. 2014. Vol. 19, No. 4. P. 53-54. Available at: https://cyberleninka.ru/article/n/kombinatsiya-metodov-lokalnoy-destruktsii-pri-pervichnom-i-metastaticheskom-rake-pecheni [In Russian].

Dronov O.I., Khomenko D.I., Bakunecj P.P., Teterina V.V. Temperaturni pokaznyky pislja kriovplyvu, potencijovanogho dystyljovanoju vodoju, na eksperymentaljnij modeli pechinky svyni za umov vidsutnosti splankhnichnogho krovotoku [Temperature changes during cryoeffect potentiated with distilled water assessed in porcine liver model without splanchnic blood flow] // Probl Cryobiol Cryomed. 2017. Vol. 27, No. 4. P. 348-355. https://doi.org/10.15407/cryo27.04.348 [in Ukrainian]

Dronov, A.I., Kovalskaja, I.A., Homenko, D.I., Zharkov, A.Ja., Leshhenko, V.M., Krutko, O.A. et al. Termometrija processa lokalnogo kriovozdejstvija v biologicheskoj tkani na diskretnyh glubinah: razrabotka kompleksa izmeritelnogo intraoperacionnogo termoparnogo chetyrehkanalnogo (KIIT-4) [Thermometry of the process of local cryoinfluence in biological tissue at discrete depths: development of a complex of measuring intraoperative four-channel thermocouple (KIIT-4)] // Hirurgija. Vostochnaja Yevropa. 2018. Vol. 7, No. 1. P. 21-27. Available at: http://surgery.recipe.by/ru/?editions=2018-tom-7-n-1-2&group_id=item_8&article_id=line_0 [In Russian]

Shafranov V.V. Mehanizmy razrushenija biologicheskih tkanej pri lokalnom kriovozdejstviі [The mechanism of destruction of biological tissue at a local cryodestruction] // Vestnik RAEN. 2012. No. 1. P. 68-77. Available at: https://elibrary.ru/item.asp?id=23715960 [In Russian]

Shchit N.M., Mіhanovskij O.A., Kazmіruk O.V. Mozhlivostі zastosuvannja nizkih temperatur v onkologіi [The capabilities of low temperature application in oncology] // Ukrainskij radіologіchnij zhurnal. 2010. Vol. 18, No. 1. P. 467-473. Available at: http://medradiologia.org.ua/assets/files/arch/2010/4/p467_473.pdf [In Ukrainian]

Zhmakin A.I. Fizicheskie osnovi kriobiologii [Physical aspects of cryobiology] // Uspehi fizicheskih nauk. 2008. Vol. 178, No. 3. P. 23-25. DOI: 10.3367/UFNr.0178.200803b.024 3 [In Russian]

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.