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nonunion of the upper lip and palate, muscle injury, satellite stem cells, engraftment, myofibrils, marker

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Kharkov , L., & Egorov , R. (2020). POSSIBILITIES OF REGENERATION OF THE MUSCLES OF THE SOFT PALATE DURING ITS NONUNION DEPENDING ON THE MYOGENIC POTENTIAL OF STEM CELLS. Review. Medical Science of Ukraine (MSU), 16(3), 63-71. https://doi.org/10.32345/2664-4738.3.2020.10


Relevance. Today there are more than 150 methods for eliminating congenital defects of the hard and soft palate. However, these techniques do not always lead to high functional results, which leads to repeated surgical interventions and long-term speech therapy rehabilitation. Therefore, there is a problem with the prognosis of such treatment. The search for a marker for assessing the prognosis of surgical intervention is relevant. One of these markers may be the state of the myogenic potential of stem cells.

Objective: to analyze the possibility of preliminary assessment of muscle regeneration, depending on the myogenic potential of stem cells, in order to increase the effectiveness of treatment of children with non-union of the soft palate.

Method. An analytical review of the literature on keywords from the scientometric databases PubMed, Scopus, Web of Science.

Results. Satellite cells represent an adequate system model for studying the biology of adult stem cells. Satellite cells can be considered candidates for cell therapy in muscle regeneration. First, they are one of the most abundant and most accessible cells in our body. Secondly, there is a panel of specific markers that can be used to isolate satellite cells. Third, satellite cells are localized within clear boundaries of the anatomical niche, and signaling mechanisms are currently being studied. Fourth, there is the possibility of recreating muscle injuries in which satellite cells can be studied. Future research aimed at increasing the purification of satellite cells so as to maintain their low differentiation, increase the engraftment potential, as well as new approaches aimed at obtaining satellite cells from iPS cells, will help accelerate the progress and development of drugs for cell therapy in the treatment of muscle degenerative diseases.

Conclusions. The data on the myogenic potential of stem cells, in muscle regeneration, obtained on satellite cell models, can be used to increase the effectiveness of the treatment of children with nonunion of the soft palate.

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1. Boldrin L., Neal A., Zammit P.S., Muntoni F., Morgan J.E. Donor satellite cell engraftment is significantly augmented when the host niche is preserved and endogenous satellite cells are incapacitated. Stem Cells. 2012 Sep; 30(9): 1971-84. DOI: 10.1002/stem.1158.
2. Conboy IM1, Rando TA. Aging, stem cells and tissue regeneration: lessons from muscle. Cell Cycle. 2005 Mar; 4(3): 407-10. DOI: https://doi.org/10.4161/cc.4.3.1518
3. Daubas P., Buckingham M.E. Direct molecular regulation of the myogenic determination gene Myf5 by Pax3, with modulation by Six1/4 factors, is exemplified by the -111 kb-Myf5 enhancer. Dev Biol. 2013 Apr 15; 376(2): 236-44. DOI: 10.1016/j.ydbio.2013.01.028.
4. Davidson E.H. Emerging properties of animal gene regulatory networks. Nature. 2010 Dec 16; 468(7326): 911-20. DOI: 10.1038/nature09645.
5. de la Garza-Rodea AS, van der Velde-van Dijke I, Boersma H, Gonçalves MA, van Bekkum DW, de Vries AA, Knaän-Shanzer S. Myogenic properties of human mesenchymal stem cells derived from three different sources. Cell Transplant. 2012; 21(1):153-73. DOI:10.3727/096368911X580554.
6. Dmitriev P, Barat A, Polesskaya A, O'Connell MJ, Robert T, Dessen P, Walsh TA, Lazar V, Turki A, Carnac G, Laoudj-Chenivesse D, Lipinski M, Vassetzky YS. Simultaneous miRNA and mRNA transcriptome profiling of human myoblasts reveals a novel set of myogenic differentiation-associated miRNAs and their target genes. BMC Genomics. 2013 Apr 18; 14:265. DOI: 10.1186/1471-2164-14-265.
7. Gilbert PM, Havenstrite KL, Magnusson KE, Sacco A, Leonardi NA, Kraft P, Nguyen NK, Thrun S, Lutolf MP, Blau HM. Substrate elasticity regulates skeletal muscle stem cell self-renewal in culture. Science. 2010 Aug 27; 329(5995): 1078-81. DOI: 10.1126/science.1191035.
8. Grefte S, Kuijpers-Jagtman AM, Torensma R, Von den Hoff JW. Skeletal muscle development and regeneration. Stem Cells Dev. 2007 Oct; 16(5): 857-68. DOI: https://doi.org/10.1089/scd.2007.0058
9. Damstra J, Mistry D, Cruz C, Ren Y. Antero-posterior and transverse changes in the positions of palatal rugae after rapid maxillary expansion. Eur J Orthod. 2009 Jun 31; 31(3): 327-32. DOI: https://doi.org/10.1093/ejo/cjn113
10. Kleinstein RN, Sinnott LT, Jones-Jordan LA, Sims J, Zadnik K. New cases of myopia in children. Collaborative Longitudinal Evaluation of Ethnicity and Refractive Error Study Group. Arch Ophthalmol. 2012 Oct; 130(10): 1274-9. DOI: https://doi.org/10.1001/archophthalmol.2012.1449
11. Kroehne V, Heschel I, Schuegner F, Lasrich D, Bartsch JW, Jockusch H. Use of a novel collagen matrix with oriented pore structure for muscle cell differentiation in cell culture and in grafts: Journal of cellular and molecular medicine. 2008; 12(5a): 1640-8. DOI: https://doi.org/10.1111/j.1582-4934.2008.00238.x
12. Lepper C1, Conway SJ, Fan CM. Adult satellite cells and embryonic muscle progenitors have distinct genetic requirements. Nature. 2009 Jul 30; 460(7255): 627-31. DOI: 10.1038/nature08209.
13. Liao YF1, Prasad NK, Chiu YT, Yun C, Chen PK. Cleft size at the time of palate repair in complete unilateral cleft lip and palate as an indicator of maxillary growth. Int J Oral Maxillofac Surg. 2010 Oct; 39(10): 956-61. DOI: 10.1016/j.ijom.2010.01.024.
14. MacQuarrie KL, Yao Z, Fong AP, Diede SJ, Rudzinski ER, Hawkins DS, Tapscott SJ. Comparison of genome-wide binding of MyoD in normal human myogenic cells and rhabdomyosarcomas identifies regional and local suppression of promyogenic transcription factors. Mol Cell Biol. 2013 Feb; 33(4):773-84. DOI: 10.1128/MCB.00916-12
15. Meligy FY1, Shigemura K, Behnsawy HM, Fujisawa M, Kawabata M, Shirakawa T. The efficiency of in vitro isolation and myogenic differentiation of MSCs derived from adipose connective tissue, bone marrow, and skeletal muscle tissue. In Vitro Cell & Dev Biol Anim. 2012 Apr; 48(4):203-15. DOI: 10.1007/s11626-012-9488-x
16. Montarras D1, Morgan J, Collins C, Relaix F, Zaffran S, Cumano A, Partridge T, Buckingham M. Direct isolation of satellite cells for skeletal muscle regeneration. Science. 2005 Sep 23; 309(5743):2064-7. DOI: https://doi.org/10.1126/science.1114758
17. Sacco A, Doyonnas R, Kraft P, Vitorovic S, Blau HM. Self-renewal and expansion of single transplanted muscle stem cells. Nature. 2008 Nov 27; 456(7221):502-6. DOI:10.1038/nature07384.
18. Soleimani VD, Punch VG, Kawabe Y, Jones AE, Palidwor GA, Porter CJ, Cross JW, Carvajal JJ, Kockx CE, van IJcken WF, Perkins TJ, Rigby PW, Grosveld F, Rudnicki MA. Transcriptional dominance of Pax7 in adult myogenesis is due to high-affinity recognition of homeodomain motifs. Dev Cell. 2012 Jun 12; 22(6): 1208-20. DOI: 10.1016/j.devcel.2012.03.014.
19. Tran S, Wang L, Le J, Guan J, Wu L, Zou J, Wang Z, Wang J, Wang F, Chen X, Cai L, Lu X, Zhao H, Guo J, Bao Y, Zheng X, Zhang T. Altered methylation of the DNA repair gene MGMT is associated with neural tube defects. J Mol Neurosci. 2012 May; 47(1):42-51. DOI: 10.1007/s12031-011-9676-2.
20. Uezumi A1, Fukada S, Yamamoto N, Takeda S, Tsuchida K. Mesenchymal progenitors distinct from satellite cells contribute to ectopic fat cell formation in skeletal muscle. Nat Cell Biol. 2010 Feb; 12(2): 143-52. DOI: 10.1038/ncb2014.
21. Qureshi IA1, Mehler MF.Emerging roles of non-coding RNAs in brain evolution, development, plasticity and disease. Nat Rev Neurosci. 2012 Jul 20; 13(8):528-41. DOI: 10.1038/nrn3234.
22. Valastyan S, Benaich N, Chang A, Reinhardt F, Weinberg RA. Concomitant suppression of three target genes can explain the impact of a microRNA on metastasis. Genes Dev. 2009 Nov 15; 23(22):2592-7. DOI: 10.1101/gad.1832709.
23. Eichhorn W, Blessmann M, Vorwig O, Gehrke G, Schmelzle R, Heiland M. Influence of lip closure on alveolar cleft width in patients with cleft lip and palate. Head & Face Medicine. 2011; 7:3. DOI:10.1186/1746-160X-7-3
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