Article PDF


chronic purulent inflammation of the lungs, rats, ozone therapy, morphology, liver

Abstract views: 31
PDF Downloads: 4

How to Cite

Alyavi , A., Sadуkova G., Rakhmatullaev , K., Tadjikhodjaeva , Y. K., & Zalyalova , Z. (2020). ОZONETHERAPY INFLUENCE ON MORPHOLOGIC CHANGES OF LIVER IN PURULENT INFLAMMATION OF THE LUNGS IN THE EXPERIMENT. Medical Science of Ukraine (MSU), 16(2), 3-8. https://doi.org/10.32345/2664-4738.2.2020.1


Relevance. Ozone increases the oxygen transport function of the blood. The use of ozone therapy for pulmonary pathology is promising. The effect of ozone therapy is associated with the ability of ozone to eliminate hypoxemia and tissue hypoxia, which is always present in patients with pathology of the bronchopulmonary apparatus. Objective: to study morphofunctional changes in liver tissue, in the dynamics of the use of ozonized water in rats in an experimental model of chronic purulent pneumonia.Materials and methods. Three groups were formed from 30 male white rats (180-200 g). In group 1 (n = 10) healthy rats were injected intraperitoneally with 5 ml of ozonated (0.02 mg / L) 0.9% NaCl once a day for 10 minutes. Course 10 days. Animals of the 2 (n = 10) and 3 (n = 10) groups were first modeled for chronic pneumonia. For this, under local novocaine anesthesia, a 1.5-2.0 cm long incision was made on the animal's neck. A nylon thread with a diameter of 0.4 mm and a length of 10-12 cm was inserted into the lumen of the trachea, between its rings, on a thin piercing needle. The distal end of the thread was located in the lumen of the trachea, and its proximal end was fixed on the skin. The wound was sutured tightly in layers. After 45 days, the thread was removed without opening the trachea. Subsequently, animals of group 2 did not receive treatment. And rats of group 3 were injected once a day with 5 ml of ozonized (0.02 mg / l) 0.9% NaCl for 10 minutes. Course 10 days. The animals were removed from the experiment by instant decapitation. The taken pieces of the liver were fixed in formalin. Histological sections were stained with hematoxylin and eosin. Microscopy was performed using an XS-213 light microscope and a Leica microscope. Results. With prolonged irritation of the respiratory tract, structural changes in the liver, characteristic of toxic hepatitis, were revealed. After treatment with ozonated saline, the morphological picture of the liver improved. In healthy rats, ozone therapy did not have a negative effect on the general condition and behavior of the animals. Conclusions. Treatment with ozonated saline improves the morphological picture of the liver of rats with chronic pneumonia.

Article PDF


1. Abdrashitova E.Kh. [Morphological characteristics of spontaneous chronic respiratory disease in rats] // Biomedicina. 2006; 1 (2). [in Russian]. http://cyberleninka.ru/article/n/morfologicheskaya-harakteristika-spontannoy-hronicheskoy-respiratornoy-bolezni-krys
2. Batyrova Z.B., Shamirzaev N.Kh. [A method for modeling chronic nonspecific pneumonia] // 2002. Patent v 20420502 а2. [in Russian].
3. Babaev Kh., Orazbaev Sh. [On the effectiveness of ozone therapy in the treatment of purulent wounds of soft tissues] // Young scientist. 2011 Sep; 9(32): 235-41. [in Russian]. https://moluch.ru/archive/32/3635/
4. Karimov M.M., Abdullaev A.Kh., Sabirova G.N. [et al] [The use of ozone therapy in the rehabilitation treatment of patients with chronic hepatitis] // Method. recom. Tashkent, 2007. 9 p. [in Russian].
5. Malenkov A.G. [Influence of ozone on the human body and the mechanisms of its therapeutic action] // Moscow, 2011. [in Russian]. http://magericmed.net/vliyanie-ozona-na-organizm-cheloveka-i-mexanizmy-ego-lechebnogo-dejstviya.html
6. Kulikov A.G. [Ozone therapy is an integral part of physiotherapy] // Physiotherapy, balneology, rehabilitation. 2005; 4: 3-7. [in Russian]. https://elibrary.ru/item.asp?id=17112525
7. Peretyagin S.P., Kostina O.V., Martusevich A.A., Didenko N.V. Influence of inhalation with reactive oxygen species on pro- and antioxidant balance in the lungs of experimental animals // Medical Almanac. 2013; 3 (27): 65-7. [in Russian]. https://cyberleninka.ru/article/n/vliyanie-ingalyatsiy-aktivnymi-formami-kisloroda-na-proi-antioksidantnyy-balans-v-legkih-eksperimentalnyh-zhivotnyh/viewer
8. Puzin S.N., Tangieva Kh.I., Zavolovskaya L.I. [Features of dynamics and structure of repeated disability due to respiratory diseases] // Mat. scientific. conf. "Equal Opportunities". M.,2009, 51-3. [in Russian].
9. Strukov P.V., Kulikov A.G., Zubkova A.V. [Normobaric hypoxia, ozone therapy and breathing exercises with expiratory resistance in the rehabilitation treatment of patients with chronic bronchitis and bronchial asthma] // Questions of balneology, physiotherapy and physiotherapy exercises. 2004; 4: 7-11. [in Russian]. http://www.fesmu.ru/elib/Article.aspx?id=113517
10. [Chernekhovskaya N.E., Shishlo V.K., Povalyaev A.V. Experimental and morphological studies of reparative regeneration of the tracheal epithelium, diffuse lymphoid tissue and regional lymph nodes under ozone therapy] // Bulletin of lymphology. 2006; 1: 34-8. [in Russian]. https://lymphology-journal.com/catalog/detail.php?SECTION_ID=762&ID=16583
11. Momot A.P., Shoikhet Ya.N. [On the role and relationship of hemostatic and inflammatory reactions in the formation of foci of purulent destruction of organs and tissues] // Problems of Clinical Medicine. 2008; 4: 102-18. [in Russian]. http://www.fesmu.ru/elib/Article.aspx?id=191787
Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.