Relevance. It is believed that when scaffolds fill in bone defects after removal of jaw cysts or revision of inflammatory foci, a new bone grows from the periphery to the center, replacing the graft and at the same time changing its properties. Delivery of all materials necessary to start the reparative process begins with the contact area of the bone bed and scaffold. The properties of the material for making the scaffold and its hydrophilicity determine its ability to incorporate chemical and cellular material from surrounding tissues.
Scaffold properties can be programmed. The properties of the fluid conductor from the wall of the bone defect to the implanted material are also important. The use of autologous blood components for this role becomes attractive.
Objective: to study the composition of autologous blood components prepared using simplified technology, and their interaction with scaffolds of the original design in the treatment of patients with destructive periodontitis and jaw cysts.
Materials and methods. Microscopic examination of the components of autologous blood and their ability to penetrate into the scaffold during surgical preparation.
Results. Microscopic examination of autologous blood after centrifugation showed a platelet count in plasma similar to the norm, increased in the liquid from the gel, as well as their presence in the gel itself and in the gel membrane. A small increase in the number of leukocytes compared to the norm is determined in the liquid obtained from the gel. In the remaining blood components, the number of leukocytes in our micropreparations is not determined. The proposed original scaffold with initial cell sizes of 10 μm during immersion in the liquid components of autologous blood showed great absorption potential against the background of the possibility of increasing its porosity.
Conclusions. The proposed simple technique for obtaining the components of autologous blood allows you to create such an environment on the border of the "bone bed-scaffold", where platelets, white blood cells and biochemical blood components are concentrated in the required quantity. On the other hand, the proposed original design of the scaffold allows, in the process of filling it with a mixture of the liquid components of autologous blood, an increase in cell sizes, which leads to an increase in the absorption volume and contact area. Both of these circumstances make it possible to achieve the necessary incorporation of biological material already before scaffold is introduced into bone defects.
2. Yegorikhina M.N. Use of blood components in fabric engineering // Siberian Medical Review. 2018. (3): 14-23. [in Russian]. DOI: https://doi.org/10.20333/2500136-2018-3-14-23
3. Kazakova V.S., Zhilyakova Ye.T., Novikov O.O. Prospects for the use of growth factors in restoration of bone tissue. Review of literature // Scientific result. 2015. 1 (3): 2015. [in Russian]. DOI: http://rrmedicine.ru/journal/article/486/
4. Kalashnikov A.V., Zubenko A.G., Rudenko I.A., Renev K.V., Rudenko R.I. First clinical experience of application of fibrin gel enriched by plate // Trauma. 2011. 12 (3). [in Russian]. URL: http://www.mif-ua.com/archive/article/23310
5. Mrtirosyan R.V. Recovery of postoperative bone defects in the jaws of osteomaterial combinations, platelet-rich plasma and the complex homeopathic preparation «Traumel' S» / Avtoreferat diss. kand. med. nauk. Moskva, 2013; 123 p. [in Russian]. URL: http://medical-diss.com/medicina/vosstanovlenie-posleoperatsionnyh-kostnyh-defektov-chelyustey-kombinatsiey-osteomaterialov-bogatoy-torombotsitami-plazmoy
6. Azzaldeen Abdulgani, Mai Abdulgani and Muhamad Abu-Hussein. Platelet-rich fibrin (PRF) in dentistry // International Journal of Applied Dental Sciences 2019; 5 (4): 01-08. URL: http://www.oraljournal.com/pdf/2019/vol5issue4/PartA/5-3-30-853.pdf
7. Balaram Naik, Karunakar P, Jayadev M. and Marshal V Rahul. Role of Platelet rich fibrin in wound healing: A critical review // J Conserv Dent. 2013. 16 (4): 284-93. DOI: https://dx.doi.org/10.4103%2F0972-0707.114344
8. Barros Mourão C.F., Gutemberg Gomes Alves. Answer controversies about hemostatic properties of platelet-rich fibrin // Oral and Maxillofacial Surgery. 2019. 23 (121): article 670. URL: https://link.springer.com/article/10.1007/s10006-018-0739-1
9. Barros Mourão C.F, Calasans-Maia M.D, de Mello Machado R.C, de Brito Resende R.F, Alves G.G . The use of platelet-rich fibrin as a hemostatic material in oral soft tissues // Oral and Maxillofac Surg. 2019. 23 (119): article 707. URL: https://link.springer.com/article/10.1007/s10006-018-0738-2
10. Beatriz Mancebo Vieira Pedro. The Effect of Platelet Rich Fibrin in Oral Surgery: A Literature Review / Dissertação Mestrado Integrado em Medicina Dentária. Lisboa, 2017/51 p. DOI: 10.13140/RG.2.2.22702.20803
11. Gaurav Vidhale, Deepali Jain, Sourabh Jain, Alkesh Vijayrao Godhane, Ganesh R. Pawar. Management of Radicular Cyst Using Platelet-Rich Fibrin & Iliac Bone Graft - A Case Report // Journal of Clinical and Diagnostic Research. 2015. 9 (6): ZD 34-6. DOI: https://doi.org/10.7860/JCDR/2015/13368.6136
12. Hartshorne Johan and Gluckman Howard. A comprehensive clinical review of Platelet Rich Fibrin (PRF) and its role in promoting tissue healing and regeneration in dentistry. Part II: Preparation, optimization, handling and application, benefits and limitations of PRF // International Dentistry – African Edition. 2016. 6 (5): 34-48. URL: http://www.moderndentistrymedia.com/oct_nov2016/hartshorne_part2.pdf
13. Reham Lotfy Aggour and Hend M. Gharib Abd El-Hady. Platelet-Rich Fibrin for the Treatment of Intrabony Periodontal Defects in Patients with Generalized Aggressive Periodontitis: A Randomized Controlled Clinical Study // Journal of the International Academy of Periodontology. 2017. 19 (1): 28-34. URL: https://www.ncbi.nlm.nih.gov/pubmed/31473710
14. Shrivastava Deepti, Srivastava Kumar Chandan. Modified Coronally Advanced Flap with Platelet Rich Fibrin as a Scaffold for Periodontal Regeneration in Gingival Recession: A Case Report // Journal of Research in Medical and Dental Science. 2019. 7 (Issue 5): 25-8. URL: https://www.jrmds.in/articles/modified-coronally-advanced-flap-with-platelet-rich-fibrin-as-a-scaffold-for-periodontal-regeneration-in-gingival-recession-a-case-44284.html
15. Tanasković Nenad. Use of Platelet-rich fibrin in maxillofacial surgery / Contemporary Materials, VII−1. 2016. P. 45-50. URL: http://doisrpska.nub.rs/index.php/conterporarymaterials3-1/article/view/4208
16. Verma Arun, Srivastava Sanjeev, Khurshid Saif, Parveen Farah, Pandey Piyush. Platelet latelet rich fibrin: A promising innovation in regenerative therapy // J of Evolution of Med and Dent Sci. 2015. 4: 5748-56. DOI: 10.14260 / jemds / 2015/840
This work is licensed under a Creative Commons Attribution 4.0 International License.