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reparative bone regeneration, scaffold, blood components

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Chumachenko , O., Topchiі D., Gromovy , Y., & Plyatsko , S. (2020). AUTOLOGOUS BLOOD COMPONENTS AND THEIR PERFORMANCE IN SCAFFOLDES MADE BY ORIGINAL TECHNOLOGY. Medical Science of Ukraine (MSU), 16(1), 40-47.


Relevance. It is believed that when scaffolds fill in bone defects after removal of jaw cysts or revision of inflammatory foci, a new bone grows from the periphery to the center, replacing the graft and at the same time changing its properties. Delivery of all materials necessary to start the reparative process begins with the contact area of ​​the bone bed and scaffold. The properties of the material for making the scaffold and its hydrophilicity determine its ability to incorporate chemical and cellular material from surrounding tissues.

Scaffold properties can be programmed. The properties of the fluid conductor from the wall of the bone defect to the implanted material are also important. The use of autologous blood components for this role becomes attractive.

Objective: to study the composition of autologous blood components prepared using simplified technology, and their interaction with scaffolds of the original design in the treatment of patients with destructive periodontitis and jaw cysts.

Materials and methods. Microscopic examination of the components of autologous blood and their ability to penetrate into the scaffold during surgical preparation.

Results. Microscopic examination of autologous blood after centrifugation showed a platelet count in plasma similar to the norm, increased in the liquid from the gel, as well as their presence in the gel itself and in the gel membrane. A small increase in the number of leukocytes compared to the norm is determined in the liquid obtained from the gel. In the remaining blood components, the number of leukocytes in our micropreparations is not determined. The proposed original scaffold with initial cell sizes of 10 μm during immersion in the liquid components of autologous blood showed great absorption potential against the background of the possibility of increasing its porosity.

Conclusions. The proposed simple technique for obtaining the components of autologous blood allows you to create such an environment on the border of the "bone bed-scaffold", where platelets, white blood cells and biochemical blood components are concentrated in the required quantity. On the other hand, the proposed original design of the scaffold allows, in the process of filling it with a mixture of the liquid components of autologous blood, an increase in cell sizes, which leads to an increase in the absorption volume and contact area. Both of these circumstances make it possible to achieve the necessary incorporation of biological material already before scaffold is introduced into bone defects.
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