Relevance. The state of dysbiosis and bacterial vaginosis (BV) is characterized by the formation of both systemic and local immune deficiency, which corresponds to the increase in the number of pathogenic microbiota. It is necessary to study the state of non-specific factors of cellular and humoral resistance in the development of bacterial dysbiosis and BV.
Objectives – to determine the state of nonspecific immunity in bacterial dysbiosis and BV on CD16-cells, as well as indicators in the blood and vaginal fluid phagocyte leukocytes activity (PhLA) and the content of the components of complement C3 and C4.
Material and methods. Data from 298 women were divided into groups according to index of pathogenic microbiota condition (IPMC) and the pathogenic microbiota indicator (PMI): normocenosis (n=53), dysbiosis I (n=128) and II degree (n=117), among the last allocated 83 patients with PMI>1 lg gE/sample, which was installed BV. Molecular genetic studies of posterolateral wall of the vagina epithelium scrapings was performed by polymerase chain reaction. Quantitatively determined by facultative and obligate anaerobic bacteria, myco- and ureaplasma, yeast-like fungi. Quantification of the cells CD16+ was performed using erythrocyte diagnosticum (LTD Granum, Ukraine). Traditional immunological methods determined by the PhLA, and components of complement C3 and C4 in blood and vaginal fluid. For statistical and regression analysis used the software Statistica 10 (StatSoft, Inc., USA).
Results. With the progression of dysbiosis has been an increase in the level of blood CD16-cells, which reached maximum at dysbiosis II degree (by 1.1-1.2 times; p≤0,005). With an increase in dysbiosis shows the formation of the phagocytosis failure, which was more common to BV and took place both at systemic and local levels (reducing the umbilical cord blood is 2.5 times 5.4 times in vaginal fluid). The complement components content were varied in the same way in the blood and vaginal fluid – increases in dysbiosis I degree and decreased with dysbiosis II degree, maximum degree, – in BV (C3 – 1.6 times in the blood and 5.0 times in vaginal secretions; p<0.001). Changes of the examined parameters was more pronounced at the local level, which contributed to the BV development.
Conclusions. With the progression of bacterial dysbiosis formed the insufficiency of non-specific immunity both at the system level and locally. Changes of the studied indicators in vaginal secretions was more pronounced, which contributed to the development of BV.
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