THE CHANGES OF HUMORAL ADRENERGIC REGULATION OF HEART IN ALCOHOL-TREATED RATS
Article PDF

Keywords

alcohol-treated rats, heart rate, β1 receptors blockade, caffeine stress, epinephrine

Abstract views: 71
PDF Downloads: 65

How to Cite

Panova, T., Koshelnyk, M., & Scherbak, S. (2017). THE CHANGES OF HUMORAL ADRENERGIC REGULATION OF HEART IN ALCOHOL-TREATED RATS. Medical Science of Ukraine (MSU), 13(1-2), 3-11. https://doi.org/10.32345/2664-4738.1-2.2017.01

Abstract

Relevance. Under the chronic influence of ethanol the genes expression is changed which contain the information about synthesis of adrenergic receptors, epinephrine enzymes. However studies of the systemic manifestations of these changes are unknown. The distortion of the adrenergic drugs effect in an alcohol-treated organism is probable.

Objective: to investigate the adrenergic regulation of the heart in alcohol-treated rates by means of variation of the number of β1 receptors and their ligands.

Materials and methods. In control (n=10) and alcohol-treated rats chronically for 120 days (n=10) the number of β1-adrenergic receptors (by means of administration of concor cor selective blocker – 0,28 mg/kg) and the quantity of epinephrine in the blood (by means of activation of sympathoadrenal system after sodium caffeine-benzoate injection – 7,14 mg/kg) were varied. The concor cor and caffeine were administered separately and together. The heart rate (HR) was recorded with the help of sphygmomanometer. The results were processed by using MedStat package.

Results. In alcohol-treated rats chronically the HR is 27,6±2,5% higher than in the control ones (383,8±12,9 min-1 and 300,8±8,7 min-1). The caffeine administration leads to an increase in heart rate by 11,5±1,1 % in control rats (up to 335,4±9,5 min-1) while it doesn’t affect this index in alcohol-treated rats (389,6±12,6 min-1). The blockade of β1 receptors leads to a decrease in the HR by 7,5±0,7 % (up to 278,2±7,9 min-1) in control rats and by 15,5±1,1 % (up to 324,4±9,3 min-1) in alcohol-treated rats. When co-administration the β1 receptors blocker and caffeine have nivelated the opposite effects of each other in the control rats and HR remained the same (307,0±8,2 min-1); this has resulted in a decrease in the HR by 4,8±1,0 % (up to 365,4±10,9 min-1) in alcohol-treated animals.

Conclusions. It is assumed that the basal secretion of epinephrine is increased and the number of β1 receptors is decreased in alcohol-treated rats compared to control ones.

https://doi.org/10.32345/2664-4738.1-2.2017.01
Article PDF

References

Adrenergicheskiye retseptory i sinapsy. URL: http://sportwiki.to/ Adrenergicheskiye_ retseptory_i_sinapsy (date of request: 07.02.2017).

Beta-adrenoblokatory. URL: http://pharmabook.net/vegetotropnye-sredstva/ (date of request: 07.02.2017).

Bortnikova A.K., Panova T.I., Kazakov V.N. Vliyaniye urovnya glikemii na potrebleniye etanola i glyukozy alkogol'zavisimymi krysami // Universitetskaya klinika. 2013. Vol. 9, No. 2. P. 169-173 [in Russian].

Yevdokimova O.V. Zavisimost' izmeneniya ekspressii rannikh genov c-fos i c-jun v miokarde krys pri kratkovremennom deystvii stressorov ot tireoidnogo statusa organizma // Zhurnal Grodnenskogo gosudarstvennogo meditsinskogo universiteta. 2014. No. 2. P. 32-36 [in Russian].

Osnovy komp'yuternoy biostatistiki: analiz informatsii v biologii, meditsine i farmatsii statisticheskim paketom MedStat / Yu.Ye. Lyakh et al. / Donetsk: Izdatel' Papakitsa Ye.K., 2006. 211 p. [in Russian].

Spisok stran po potrebleniyu alkogolya na cheloveka. VOZ. 2011. URL: http://dic.academic.ru/ (date of request: 07.02.2017).

β1-Adrenergic Receptor Signaling Activates the Epithelial Calcium Channel, Transient Receptor Potential Vanilloid Type 5 (TRPV5), via the Protein Kinase A Pathway / E.A.E. van der Hagen et al. // J. Biol. Chem. 2014. Vol. 289, No. 26. Р. 18489-18496.

Amanfu R.K., Saucerman J.J. Modeling the Effects of β1-Adrenergic Receptor Blockers and Polymorphisms on Cardiac Myocyte Ca2+ Handling // Mol. Pharmacol. 2014. Vol. 86, No. 2. Р. 222-230.

A Systematic Review and Meta-Analysis of Long-Term Outcomes After Septal Reduction Therapy in Patients With Hypertrophic Cardiomyopathy / M. Liebregts et al. // JACC Heart Fail. 2015. Vol. 3, No. 11. P. 896-905.

Burnstock G. Purinergic signalling in endocrine organs // Purinergic Signal. 2014. Vol. 10, No. 1. Р. 189-231.

Caffeine-Induced Activated Glucocorticoid Metabolism in the Hippocampus Causes Hypothalamic-Pituitary-Adrenal Axis Inhibition in Fetal Rats / D. Xu et al. // PLoS One. 2012. Vol. 7, No. 9. e 44497. URL: www.ncbi.nlm.nih.gov/pubmed/ (date of request: 07.02.2017).

Caffeine Inhibits the Activation of Hepatic Stellate Cells Induced by Acetaldehyde via Adenosine A2A Receptor Mediated by the cAMP/PKA/SRC/ERK1/2/P38 MAPK Signal Pathway / H. Wang et al. // PLoS One. 2014. Vol. 9, No. 3. e 92482. URL: www.ncbi.nlm.nih.gov/pubmed/ (date of request: 07.02.2017).

Chopra K., Tiwari V. Alcoholic neuropathy: possible mechanisms and future treatment possibilities / // Br. J. Clin. Pharmacol. 2012. Vol. 73, No. 3. P. 348-362.

Chronic stress enhances progression of periodontitis via α1-adrenergic signaling: a potential target for periodontal disease therapy / L. Huaixiu, X. Minguang, W. Feng // Exp. Mol. Med. 2014. Vol. 46, No. 10. e118. URL: www.ncbi.nlm.nih.gov/pubmed/ (date of request: 07.02.2017).

Different mechanisms for histone acetylation by ethanol and its metabolite acetate in rat primary hepatocytes / S.D. Shukla et al. // J. Pharmacol. Exp. Ther. 2015. Vol. 354, No. 1. P. 18-23.

DNA co-methylation modules in postmortem prefrontal cortex tissues of European Australians with alcohol use disorders / F. Wang et al. // Sci. Rep. 2016. Vol. 6. e19430. URL: www.ncbi.nlm.nih.gov/pubmed/ (date of request: 07.02.2017).

Fitzgerald P.J. Elevated Norepinephrine may be a Unifying Etiological Factor in the Abuse of a Broad Range of Substances: Alcohol, Nicotine, Marijuana, Heroin, Cocaine, and Caffeine // Subst. Abuse. 2013. No.7. Р. 171-183.

FOLFOX chemotherapy as a cause of ventricular septal rupture after alcohol septal ablation for obstructive hypertrophic cardiomyopathy? / M. Liebregts et al. // Int. J. Cardiol. 2016. Vol. 207. P. 208-210.

Heart ventricles specific stress-induced changes in β-adrenoceptors and muscarinic receptors / A. Tillinger, M. Novakova, O. Krizanova // Gen. Physiol. Biophys. 2014. Vol. 33, No. 3. P. 357-364.

Higley A.E., Koob G.F., Mason B.J. Treatment of Alcohol Dependence With Drug Antagonists of the Stress Response // Alcohol. Res. 2012. Vol. 34, No. 4. Р. 516-521.

Histone Deacetylase Gene Expression Following Binge Alcohol Consumption in Rats and Humans / J.A. López-Moreno et al. // Alcohol. Clin. Exp. Res. 2015. Vol. 39, No. 10. P. 1939-1950.

Increased DNA methylation in the livers of patients with alcoholic hepatitis H / Shen et al. // Exp. Mol. Pathol. 2015. Vol. 99, No. 2. P. 326-329.

Klatsky A.L. Alcohol and cardiovascular diseases: where do we stand today? Review // J. Intern. Med. 2015. Vol. 278, No. 3. P. 238-250.

Kyzar E.J., Pandey S.C. Molecular mechanisms of synaptic remodeling in alcoholism // Neurosci. Lett. 2015. No. 601. P. 11-19.

Mustard J. A. The buzz on caffeine in invertebrates: effects on behavior and molecular mechanisms // Cell Mol. Life Sci. 2014. Vol. 71, No. 8. Р. 1375-1382.

Natarajan S.K., Pachunka J.M., Mott J.L. Role of microRNAs in Alcohol-Induced Multi-Organ Injury. Review // Biomolecules. 2015. Vol. 5, No. 4. P. 3309-3338.

Olini N., Kurth S., Huber R. The Effects of Caffeine on Sleep and Maturational Markers in the Rat // PLoS One. 2013. Vol. 8, No. 9. e 72539. URL: www.ncbi.nlm.nih.gov/pubmed/ (date of request: 07.02.2017).

Reductions in the cardiac transient outward K+ current Ito caused by chronic β-adrenergic receptor stimulation are partly rescued by inhibition of nuclear factor kappaB / B.K. Panama et al. // J. Biol. Chem. 2016. Vol. 291, No. 8. P. 4156-4165.

Resilience to Meet the Challenge of Addiction: Psychobiology and Clinical Considerations / T.N. Alim et al. // Alcohol Res. 2012. Vol. 34, No. 4. Р. 506-515.

The effect of caffeine on glucose kinetics in humans – influence of adrenaline / D.S. Battram et al. // J. Physiol. 2005. Vol. 569, Pt. 1. Р. 347-355.

Transcoronary ethanol ablation for incessant ventricular tachycardia: a salvage technique when faced with left ventricular thrombus / A. Frontera et al. // Neth. Heart J. 2015. Vol. 23, No. 11. P. 555-556.

Up-Regulation and Functional Effect of Cardiac β3-Adrenoreceptors In Alcoholic Monkeys / H.-J. Cheng et al. // Alcohol Clin. Exp. Res. 2010. Vol. 34, No. 7. P. 1171-1181.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.