THROMBOSE-PREVENTIVE INFLUENCE OF QUERCETIN IN RATS ON THE HYPER-COAGULATION DIET
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Keywords

quercetin, hyper-coagulation diet, hemostasis

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Potaskalova, V., Khaitovych, M., Plenova, O., & Valigura, M. (2019). THROMBOSE-PREVENTIVE INFLUENCE OF QUERCETIN IN RATS ON THE HYPER-COAGULATION DIET. Medical Science of Ukraine (MSU), 15(3-4), 19-23. https://doi.org/10.32345/2664-4738.3-4.2019.03

Abstract

Relevance. Hyper-coagulation processes increase the risk of coronary heart disease.

Objective of the study was to study the effect of quercetin on the hemostatic system when using hyper-coagulation diet in rats.

Materials and methods. The study included 17 male rats weighing 240-270 g. The rats of the control group (n = 6) were fed with conventional compound feed; Group I rats (n = 5) - mixed feed; and group II rats (n = 6) mixed with meat and quercetin (1.33 g/l). After 21 days, sterile blood was collected. The Amellung Coagulometer KS 4A studied the external and internal blood coagulation pathways, the activity of their own anticoagulant and fibrinolytic blood systems. The data obtained were statistically processed using Excel 2010 and Statistica v.10 (StatSoft, USA).

Results. In rats of group 1, the external pathway of blood coagulation underwent the most significant changes, as indicated by an almost four-fold acceleration of prothrombin time and a decrease in the international normalized ratio. From the side of the internal pathway, the greatest changes were recorded in the last phase of clot formation: thrombin time decreased by 39.8% with an almost constant rate of activated partial thromboplastin time (APTT), which confirmed the activation of the fibrin formation phase. In addition, the content of soluble fibrin-monomer complexes (RFMC) increased 1.7 times and the fibrinogen decreased. At the same time, depletion of the anticoagulant potential of the blood was observed (a 42.2% decrease in the activity of the protein C system with a tendency to a decrease in antithrombin III (AT III). The fibrinolytic system was significantly suppressed, as indicated by more than three times inhibition of XII-dependent fibrinolysis. In animals Group 2 compared with the indicator in group 1, APTT was 2 times longer; the concentration of RFMC was also doubled, fibrinolysis was activated by 25%.

Conclusion: The use of quercetin bioflavonoids in animals with simulated coronary heart disease slows down blood coagulation and potentiates the anticoagulant system, including due to a 40% increase in AT III activity, can be considered as a thrombosis-preventive effect

https://doi.org/10.32345/2664-4738.3-4.2019.03
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References

1. Galyautdinov G.S., Chudakova E.A. Features of haemostasis in patients with coronary heart disease // Kazan Medical Journal. 2012; 93 (1): 3-7. [in Russian]. https://doi.org/10.17816/KMJ2134. URL: https://kazanmedjournal.ru/kazanmedj/article/view/2134
2. Kamenev V.F., Strelnikova I.L., Maslennikov A.A. Comparative characteristics of hemostasis system of aorta and veins in patients with chronic cardiac insufficiency caused by chronic cardiac insufficiency // Scientific reports of Belgorod State University. 2012; 4 (123): 81-4. [in Russian]. URL: https://www.bsu.edu.ru/upload/iblock/09a/u4e123l%20wvd%2017.pdf
3. Karpenko O.O. Condition of coagulation, anticoagulant and fibrinolytic activity of blood in patients with acute coronary heart disease and peculiarities of changes in combination with type 2 diabetes (cohort prospective study // Scientific Journal «ScienceRise: Medical Science». 2019; 4 (31): 4-8. [in Ukrainian]. https://doi.org/10.15587/2519-4798.2019.174303. URL: http://journals.uran.ua/sr_med/article/view/174303
4. Ionova Zh.I., Berkovich O.A., Kostareva A.A., Pchelina S.N., Sergeeva E.G. Pathogenetic mechanisms of immune inflammation of the vascular wall: the role of peroxisome proliferation activator receptors a and in types and tissue factor // Translational Medicine.2015; 2-3: 18-22. [in Russian]. https://doi.org/10.18705/2311-4495-2015-0-2-3-18-22. URL: https://transmed.almazovcentre.ru/jour/article/view/63/64#
5. Connor W.E., Connor S.L. Diet, atherosclerosis, and fish oil // Adv Intern Med. 1990; 35: 139-71. https://www.ncbi.nlm.nih.gov/pubmed/2405591
6. Dabeek W.M., Marra M.V. Dietary Quercetin and Kaempferol: Bioavailability and Potential Cardiovascular-Related Bioactivity in Humans // Nutrients. 2019; 11 (10): pii: E2288. doi: 10.3390/nu11102288.
7. Yu P.X., Zhou Q.J., Zhu W.W., et al. Effects of quercetin on LPS-induced disseminated intravascular coagulation (DIC) in rabbits // Thromb Res. 2013; 131 (6): e270-273. doi: 10.1016/j.thromres.2013.03.002.
8. Wongchitrat P., Klosen P., Pannengpetch S., et al. High-fat diet-induced plasma protein and liver changes in obese rats can be attenuated by melatonin supplementation // Nutr Res. 2017; 42: 51-63. doi: 10.1016/j.nutres.2017.04.011.
9. Riva A., Corti A., Belcaro G., et al. Interaction study between antiplatelet agents, anticoagulants, diabetic therapy and a novel delivery form of quercetin // Minerva Cardioangiol. 2019; 67 (1): 79-83. doi: 10.23736 / S0026-4725.18.04795-3.
10. Lippi G., Cervellin G., Mattiuzzi C. Red meat, processed meat and the risk of venous thromboembolism: friend or foe? // Thromb Res. 2015; 136 (2): 208-11. doi: 10.1016/j.thromres.2015.04.027.
11. Magar R.T., Sohng J.K. A review on structure, modifications and structure-activity relation of quercetin and its derivatives // J Microbiol Biotechnol. 2019. doi: 10.4014/jmb.1907.07003. URL: http://www.jmb.or.kr/journal/paper_view.html?book=&tops=&start=0&scale=15&key=&key_word=&Vol=&Num=&PG=&year1=&year2=&sort=&aut_box=&sub_box=&sos_box=&key_box=&pub_box=&abs_box=&mod=&mnum=5956
12. Mosawy S., Jackson D.E., Woodman O.L., et al. The flavonols quercetin and 3',4'-dihydroxyflavonol reduce platelet function and delay thrombus formation in a model of type 1 diabetes // Diab Vasc Dis Res. 2014; 11 (3): 174-81. doi: 10.1177 / 1479164114524234. URL: https://journals.sagepub.com/doi/full/10.1177/1479164114524234
13. Yamagata K. Polyphenols Regulate Endothelial Functions and Reduce the Risk of Cardiovascular Disease / K. Yamagata // 2019; 25 (22): 2443-58. doi: 10.2174/1381612825666190722100504.
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