Relevance. The chronic stress influences on increasing of frequency of thyroid pathology together with other factors (decreased iodine intake in food, harmful factors of external environment, high technogenic loading, depravation of general ecological state, violation of structure of nutrition). The changes of hypothalamus-hypophysis-thyroid gland axis at stress is not enough agreed because of influence stressful factors with different character and duration.
Objective is study of influence of chronic emotional painful stress (CS) on the changes of morphofunctional state of thyroid gland (TG) on the background of experimental hypothyroidism (EH).
Materials and methods. Experiments were performed on 48 white laboratory sex matured male rats. Animals were divided on four groups: the fist – intact animals; the second – animals with EH; the third – animals under influence of CS; the fourth – animals with EH under action of CS. The EH was modeled by everyday addition of mercazolilum (dose 10mg/kg) to drinking water during 15 days. The CS was modeled by 1 hour per day during 30 days. The functional activity of TG was estimated by the concentration of thyroid stimulating hormone (TSH), triiodothyronine (T3), thyroxin (T4) in blood plasma and calculation of thyroid index (TI). The morphological state of TG was studied by light and electron microscopy. The statistical analysis of results was processed using Student’s test with Bonferroni-Holm correction in program Statistica 6.0. The differences were statistical significant at р<0,05.
Results. The long-term influence of stress factors caused significant decreasing of level of thyroid hormones in blood plasma in comparison to intact animals. The data of light and electron microscopy of TG of rats under influence of CS confirm biochemical changes and detect signs of decreasing of secretory function. On the background of action of mercazolilum the long-term stress caused to more substantial changes of functional state of TG (significant increasing of level of TSH, decreasing of content of T4 and TI) in comparison to data of animals with EH. The structural features of TG of animals with EH under influence of CS indicate on suppression of function.
Conclusions. Decreasing of synthesis of hormones by TG in rats at conditions of CS was detected. The long-term action of stress on background of hypothyroidism causes to more substantial depravation of thyroid profile.
Bobyrova L.E., Dvornyk I.L., Muravlova O.V., Horodynska O.Yu. Diagnostic and treatment of endocrine emergencies. - Poltava: 2017. 99 p. [Ukrainian]
Detsyk O.Z. Methodological approach to summation of scientific research results // Galician Medical Journal. 2011; 18 (2): 5-8. [Ukrainian]
Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes // Official Journal of the European Union. 2010; L276: 33-79. https://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2010:276:0033:0079:en:PDF
Shields G.S., Kuchenbecker S.Y., Pressman S.D., Sumida K.D., Slavich G.M. Better cognitive control of emotional information is associated with reduced pro-inflammatory cytokine reactivity to emotional stress // Stress. 2016; 19 (1): 63-8. doi: 10.3109/10253890.2015.1121983
Herasymchuk M.R. Manifestations of experimental hypothyroidism during the influence of moderate physical activity // Pathology. 2015; 1 (33): 80-3. [Ukrainian]
Kononenko A.H., Kravchenko V.M. Histological study of thyroid state in administration of aqueous extract from lemna minor frond in experimental hypothyroidism // Medical and clinical chemistry. 2016; 18 (2): 10-6. [Ukrainian] http://www.irbis-nbuv.gov.ua/cgi-bin/irbis_nbuv/cgiirbis_64.exe?Z21ID=&I21DBN=JRN&P21DBN=JRN&S21STN=1&S21REF=10&S21FMT=njuu_all&C21COM=S&S21CNR=20&S21P01=0&S21P02=0&S21COLORTERMS=0&S21P03=I=&S21STR=%D0%96101293/2016$
Nebozhina M.V. The influence of experimental hypothyroidism induced by mercasolil on state of processes of free-radical oxidation in hepatic tissue in conditions of X-ray irradiation // Endocrinology. 1999; 4 (2): 261. [Russian]
Petryk H.V., Hnat S.V. Morphological and functional changes of thyroid body in iodine deficiency area in women of reproductive age // Nursing. 2014. (1): 61-3. [Ukrainian] http://medlib.dp.gov.ua/jirbis2/images/fond_publications/medsestrinstvo/medsestrinstvo_1_2014.pdf
Vadzyuk Yu.S. Optimization of pharmaceutical provision for patients with thyroid pathologies: thesis of manuscript for obtaining the degree of candidate of pharmaceutical sciences (PhD) by specialty: 15.00.01 “Drug technology, organization of pharmaceutical business and judicial pharmacy”. Lviv, 2017. 28 p. [Ukrainian]
Valov S.D. The neurobiological aspects of regulation of proliferation, growth and cell differentiation of gland epitheliums different genesis: thesis of manuscript for obtaining the degree of doctor of medical sciences by specialty: 03.00.25 “Histology, cytology, cell biology”. Tomsk, 2004. 40 p. [Russian]
Xiaoxiao Lin, Christy A. Itoga, Sharif Taha, Ming H. Li, Ryan Chen, Kirolos Sami and oth. C-Fos mapping of brain regions activated by multimodal and electric foot shock stress // Neurobiology of Stress. 2018. 8: 92-102. DOI: 10.1016/j.ynstr.2018.02.001
This work is licensed under a Creative Commons Attribution 4.0 International License.