THEORETIC AND EXPERIMENTAL SUBSTANTIATION OF A COMPOSITION OF A CREAM WITH CERIUM DIOXIDE NANOPARTICLES
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Keywords

cream with cerium dioxide nanoparticles, screening, photoprotective action, photodynamic injury

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Zaychenko, G., Liapunov, M., Iefanov, V., Bezuhla, O., Liapunova, A., & Simonova, O. (2019). THEORETIC AND EXPERIMENTAL SUBSTANTIATION OF A COMPOSITION OF A CREAM WITH CERIUM DIOXIDE NANOPARTICLES. Medical Science of Ukraine (MSU), 15(1-2), 3-11. https://doi.org/10.32345/2664-4738.1-2.2019.01

Abstract

Relevance. Cutaneous malignancies are one of the most widespread form of cancer with increasing prevalence and Ukraine and worldwide. A negative impact of ultraviolet radiation is one of the risk factors of this pathology, and it can be prevented by photoprotectors. It is expedient to develop a new effective and safe sunscreen with cerium dioxide nanoparticles, which is deprived of photocatalytic properties and has prominent photoprotective and antioxidant action.

Objective: theoretic and experimental substantiation of a composition of a cream with cerium dioxide nanoparticles by the means of a screening of photoprotective action on a model of a photodynamic injury in guinea pigs with further identification of a leader cream.

Material and methods. A photodynamic injury in guinea pigs was modeled with ultraviolet emitter with an exposition of 5 minimal erythema doses. Twenty minutes prior to exposure a cream with 0.1%, 0.25% or 0.5% cerium dioxide nanoparticles or a comparator cream with 3% titanium dioxide was applied topically. A leader cream was then chosen based on the following criteria: photoprotective action, skin temperature, blood leukocyte count, and serum histamine level.

Results. The cream with 0.25% cerium dioxide nanoparticles was identified as a leader cream, because its photoprotective action was 43.6%, compared to 23.1% and 35.9% for 0.1% and 0.5% creams, respectively. After an application of the cream with 0.25% cerium dioxide nanoparticles there were less ulcers and deep skin lesions and 17.4% less leukocytosis than in pathology control group; the lowest increase (0.16 °С) in skin temperature was observed in comparison with other test creams; no increase in serum histamine level was detected.

The cream with 0.25% cerium dioxide nanoparticles was more effective than a comparator. Four hours post-exposure there was 57.1% less pronounced erythema in the leader cream group than in the cream with 3% titanium dioxide group. photoprotective action of a comparator was only 23.1%. Skin temperature in a comparator group 4 hours post-exposure was 0.26 °С higher than in the cream with 0.25% cerium dioxide nanoparticles group. Moreover, 8.6% more pronounced leukocytosis was observed in the cream with 3% titanium dioxide group, compared to the leader cream group.

Conclusions. The cream with cerium dioxide nanoparticles 0.25% was recognized as the leading sample and recommended for choosing technology and final composition of the finished dosage form, for further in-depth pharmacological study by totality of studied parameters.

https://doi.org/10.32345/2664-4738.1-2.2019.01
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References

Bulletin of the National Cancer Registry No. 19 - Cancer in Ukraine, 2016-2017 / Ed. OO Kolesnik / Kyiv: National Cancer Institute, 2018. 136 p. [Ukrainian] http://www.ncru.inf.ua/publications/BULL_19/index.htm

Preclinical drug research: guidelines / ed. OV Stefanov. Kiev: Avicenna, 2001. 528 p. [Ukrainian] https://www.twirpx.com/file/537410/

Nanocrystalline cerium dioxide: properties, production, application / V.K. Ivanov et al. Tomsk: Tomsk University Publishing House, 2013. 284 p. [Russian] https://www.researchgate.net/profile/Alexander_Shcherbakov6/publication/273205483_NANOCRYSTALLINE_CERIUM_DIOXIDE_properties_synthesis_applications/links/54fbf9590cf20700c5e85dee/NANOCRYSTALLINE-CERIUM-DIOXIDE-properties-synthesis-applications.pdf

Pathological physiology: textbook. / NN Zaiko et al. Moscow: MEDPress-Inform, 2007. 640 p. [Russian]

Directory. Physiological, biochemical and biometric parameters of experimental animals / Ed. V.G. Makarov, MN Makarova. St. Petersburg: Lemma, 2013. 116 p. [Russian] http://doclinika.ru/wp-content/uploads/2016/02/SPRAVOCHNIK.-Fiziologicheskie-biohimicheskie-i-biometricheskie-pokazateli-normy-e-ksperimental-ny-h-zhivotny-h.pdf

Caputo F, De Nicola M, Sienkiewicz A, Giovanetti A, Bejarano I, Licoccia S, Traversa E, Ghibelli L. Cerium oxide nanoparticles, combining antioxidant and UV shielding properties, prevent UV-induced cell damage and mutagenesis // Nanoscale. 2015; 7 (38): P. 15643-56. doi: 10.1039/c5nr03767k.

Guy G.P., Ekwueme D.U. Years of potential life lost and indirect costs of melanoma and non-melanoma skin cancer: a systematic review of the literature // Pharmacoeconomics. 2011; 29 (10): 863-74. doi: 10.2165/11589300-000000000-00000.

Wu S, Cho E, Li WQ, Weinstock MA, Han J, Qureshi AA. History of severe sunburn and risk of skin cancer among women and men in 2 prospective cohort studies // Am. J. Epidemiol. 2016. 183 (9): 824-33. doi: 10.1093/aje/kwv282.

Ruszkiewicz JA, Pinkas A, Ferrer B, Peres TV, Tsatsakis A, Aschner M. Neurotoxic effect of active ingredients in sunscreen products, a contemporary review / J. A. Ruszkiewicz et al. // Toxicol. Rep. 2017; 4: 245-59. doi: 10.1016/j.toxrep.2017.05.006. eCollection 2017.

Pasparakis M., Haase I., Nestle F.O. Mechanisms regulating skin immunity and inflammation // Nat. Rev. Immunol. 2014; 14 (5): 289-301. doi: 10.1038/nri3646.

Martens MC, Seebode C, Lehmann J, Emmert S. Photocarcinogenesis and skin cancer prevention strategies: an update // Anticancer Res. 2018; 38 (2): 1153-8. https://doi.org/10.21873/anticanres.12334

Li Y, Li P, Yu H, Bian Y. Recent advances (2010-2015) in studies of cerium oxide nanoparticles’ health effects // Environ. Toxicol. Pharmacol. 2016; 44: 25-9. https://doi.org/10.1016/j.etap.2016.04.004

Stiefel C., Schwack W. Photoprotection in changing times – UV filter efficacy and safety, sensitization processes and regulatory aspects // Int. J. Cosmet. Sci. 2015; 37 (1): 2-30. https://doi.org/10.1111/ics.12165

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